Technical Data
Cooked Meat Medium (Powder)
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Beef Heart454
Proteose Peptone20
Sodium Chloride5
Suitable for the cultivation of anaerobes and stock culture maintenance. Used to differentiate saccharolytic from proteolytic Clostridium sp. Saccharolytic species form acid and gas without digesting the meat. Proteolytic species form sulfur compounds (putrid smell) and break down the meat to amino acids (blackening). Also suitable for recovery of Clostridium botulinum from foods.
Expected cultural response after 48-72 hours at 35.0C.

Place 1.25g of meat granules into a test tube. Add 1ml purified water. Autoclave at 121C for 15 minutes.

Directions: 1. Dissolve 1.25grams per ml of distilled/deionized (DDI) water.

2. Autoclave for 15 minutes at 121C (15psi) Storage:Dehydrated medium may be stored at 2-30C. After opening container, store recapped at same temperature in low humidity environment. Discard if not free flowing or if the color has changed from the original brown pellets. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Brown pellets

Solubility: Medium amber supernatant, insoluble granules

pH (25C): 7.20.2

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.