Technical Data
C7901-36A
Coronin 1 (CLABP, CLIPINA, CO1A, CORO1A, Coronin actin binding protein 1A, Coronin like protein A, Coronin like protein p57, HCORO1, p57, TACO, Tryptophan aspartate containing coat protein, Clabp TACO, Coronin actin binding protein, 1A, Coronin-1)
Description:
Coronins are actin-binding proteins in eucaryotic cells. The prototype of coronin were found in Dictyostelium discoideum. The protein is involved in many cell processes, e.g. phagocytosis, cytokinesis and migration.

Cellular Localization: In noninfected macrophages, associated with the cortical microtubule network; in mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification.

Positive Control: MOLT-4, Jurkat

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide: KRLDRLEETVQAK, representing the C-Terminus of the human protein (residues 449-461), according to NP_009005.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
Specificity:
Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) and bovine (100% identity with immunogen) due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Ferrari G et al. A coat protein on phagosomes involved in the intracellular survival of mycobacteria. Cell 97:435-47 (1999).