		Technical Data
C7901-80	Cortactin (p80,85)

Description:
Cortactin is present as a p80 and p85kD protein in many cell types. The protein was first identified as being a major substrate of the tyrosine kinase pp60SRC. The main structural feature of the protein is that at the N-terminus there are five and a half 37-amino acid repeats, these appear to be responsible for the actin-binding. This repeat region occupies about a third of the protein. The repeat region is followed by a helical region and then a proline rich region and finally a SH3 domain.

Applications:
Suitable for use in Western Blot, Immunoprecipation and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5-2ug/ml detects Cortactin (p80/85) in mouse 3T3/A31 cell lysates. Detects Cortactin in chicken fibroblast src-transformed cell lysate. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C7901-80. Proteins were visualized using an IgG Gt x Mo (HRP) and a chemiluminescence detection system.
Immunoprecipitation: Use 5ug for ~500ug of cell lysate.
Immunocytochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma x AJ mouse splenocytes

Included Positive Antigen Control:
F4209: 3T3/A31 cell lysate. Add 2.5ul 2-mercaptoethanol/100ul of lysate. Boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane.
Note: PC-12 cell lysate, HeLa whole cell lysate or A431 whole cell lysate can also be used as a positive control.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	0.T.21	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Chicken	Mouse

Concentration:

~1mg/ml

Immunogen:

Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60 src.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

Specificity:

Recognizes 80kD and 85kD Cortactin protein in chicken and avian cells. Recognizes 80kD protein in rodent and human cells. Species Crossreactivity: mouse, rat, hamster and bovine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Parsons, et al., Proc. Natl. Acad. Sci. 87: 3328 (1990). 2. Parsons, et al., Mol. Cell Biol. 11: 5113 (1991). 3. Hoffman, I., et al., J. Cell Biol. 155: 133-143 (2001). 4. Lavoie, J.N., et al., J. Cell Biol. 150: 1037-1056 (2000). 5. McNiven, M.A., et al., J. Cell BIol. 151: 187-198 (2000). 6. Katsube, T., et al., J. Biol. Chem. 273: 29,672-29,677 (1998). 7. Kim, L., et al., J. Biol. Chem. 273: 23,542-23,548 (1998).