Technical Data
Cortactin (p80/85 src substrate, Src substrate cortactin, Amplaxin, CTTN, EMS1, Mammary tumor and squamous cell carcinoma associated, Oncogene EMS1)
Cortactin is an 80/85kD cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha-helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr-421, Tyr-470, and Tyr-486). In vitro, cortactin binds to and cross-links F-actin into meshworks.

Cellular Localization: Cytoplasmic

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide with sequence WKASAGHAVSIA representing the N terminus of the human protein (residues 2-13) according to NP_005222.
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse, Rat due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Schuuring E et al. Identification and cloning of two overexpressed genes, U21B31/PRAD1 and EMS1, within the amplified chromosome 11q13 region in human carcinomas. Oncogene 7:355-61 (1992).