Technical Data
C7901-85G
Cortactin (CTTN)
Description:
Cortactin is an 80/85kD cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37aa sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an a-helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr-421, Tyr-470, and Tyr-486). In vitro, cortactin binds to and cross-links F-actin into meshworks.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry:1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceMouseRabbit
Concentration:
As reported
Immunogen:
Synthetic non-phosphopeptide corresponding to mouse Cortactin around the phosphorylation site of tyrosine 421 (P-I-YP-E-D).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes mouse Cortactin. Species Cross-Reactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.