Technical Data
Cortactin (Src Substrate Cortactin, EMS1, Amplaxin, CTTN, Oncogene EMS1)
Cortactin is a c-src substrate associated with sites of dynamic actin assembly at the leading edge of migrating cells promotes branched actin filament network formation by Arp2:3 complex. Cortactin is an F-actin-binding protein. The amino-terminal half of the protein is comprised of multiple copies of an internal, tandem 37aa repeat while the carboxyl-terminal half contains a distal SH3 domain. Cortactin, present in several different cell types, is enriched in cortical structures such as membrane ruffles and lamellipodia. The properties of cortactin indicate its importance for microfilament-membrane interactions as well as transducing signals from the cell surface to the cytoskeleton. Human cortactin gene is localized on chromosome 11q13.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.01-0.03ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse and Rat Brain lysates

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide corresponding to WKASAGHAVSIA-C, from the N-terminus of the protein sequence according to NP_005222.2; NP_612632.1.
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Recognizes mouse Cortactin. Species sequence homology: bovine, canine, human, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schuuring E, Verhoeven E, Mooi WJ, Michalides RJ. Identification and cloning of two overexpressed genes, U21B31/PRAD1 and EMS1, within the amplified chromosome 11q13 region in human carcinomas. Oncogene 1992 Feb;7(2):355-61 PMID: 1532244.