Technical Data
C7901-86
Cortactin, phosphorylated (Tyr466)
Description:
Cortactin is an 80/85kD cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37aa sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an a helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are Src phosphorylation sites at tyrosines 421, 466 and 482 (421, 470 and 486 in the human sequence) that when phosphorylated, attenuate the cross-linking of F-actin into meshworks.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
10 blots-20°CBlue IceMouseRabbit
Concentration:
Not determined
Immunogen:
Synthetic phosphopeptide corresponding to mouse cortactin that contains tyrosine 466 (tyrosine 470 in the human sequence).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH7.3, 1mg/mL BSA (IgG, protease free), 0.05% sodium azide.
Specificity:
Recognizes mouse cortactin phosphorylated at Tyr466. Species sequence homology: human (62%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dudek, S.M., et al. (2002) Biochem. Biophys. Res. Comm. 29 (4):511-519. 2. Arderiu, G., et al. (2002) Biochem. J. 364(Pt 1):65-71. 3. Weed, S.A. and J.T. Parsons (2001) Oncogene 20(44):6418-6434. 4. Weed, S.A., et al. (2000) J. Cell. Biol. 151(1):29-40. 5. Kapus, A., et al. (2000) J. Biol. Chem. 275(41):32289-32298. 6. Huang, C., et al. (1998) J. Biol. Chem. 273(40):25770-25776.