Technical Data
C7904-73J
COX 1 (Cyclooxygenase-1, PGHS-1, Prostaglandin Endoperoxide Synthase-1, PHS 1, Prostaglandin H2 synthase 1)
Description:
Cyclooxygenase (COX), also known as prostaglandin H2 synthese and prostaglandin endoperoxide synthase, is an important enzyme in the conversion of arachidonic acid to prostaglandin H2.1, 2 Prostaglandin H2 is converted by other enzymes into inflammatory mediators such as prostaglandin (PG) D2, PGE2, PGF2a, PG12, and thromboxane A2. Thus, COX is a key enzyme in the production of inflammatory agents and is the target of intense research and drug discovery activities. COX consists of two isoforms, COX-I (599 amino acid residues) and COX-II (604 amino acid residues).

The COX enzymes, membrane-associated heme proteins that have cyclooxygenase and peroxidase activities, are targets of NSAID (nonsteroidal anti-inflammatory drugs) such as aspirin.3 COX-I is involved in the regulation of homeostatic functions throughout the body, such as vascular hemostasis, renal blood flow, and maintenance of glomerular function.4, 5, 6 It is constitutively produced in most cell types with high expression levels found in gastrointestinal tissues. COX-I is important in the gut for the production of prostaglandins, which inhibit gastric secretion. COX-I can be induced in monocytes, macrophages, and other cells as part of the inflammatory response by IL-1b and other cytokines. It is also induced by growth factors such as EGF and PDGF. Expression is inhibited by glucocorticosteroids such as cortisol and dexamethasone.

Cellular Localization: Cytoplasmic

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18J1067Affinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Full length human COX-1.
Purity:
Purified by Protein A affinity chromatography.
Form
0.08% Sodium Azide PBS, pH 7.4
Specificity:
Detects a band at approximately 70kD. Species Crossreactivity: Crossreacts with Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Vane, J.R., and Botting, R.M., Inflamm. Res., 44, 1 (1995).
2. Smith, W.L., et al., In: Intracellular Messengers. Taylor, C.W. ed., London, Pergamon Press, p. 101 (1993).