Technical Data
COX 1 (Cyclooxygenase 1, Cyclooxygenase-1, COX-1, COX1, Prostaglandin Endoperoxide Synthase-1, Prostaglandin G/H Synthase 1, PGH Synthase 1, PGHS1, PGHS-1, Prostaglandin G/H Synthase and Cyclooxygenase, Prostaglandin H Synthase 1, PHS 1, PHS1, PHS-1, Pros
Cyclooxygenase 1 (COX1), also known as Prostaglandin-endoperoxide synthase (PTGS1), is the key enzyme in prostaglandin biosynthesis. The gene was approximately 40kb long, with 11 protein-coding exons. There were 599aa residues with a calculated molecular mass of approximately 68kD. By analysis of a human/hamster somatic hybrid DNA panel, Funk et al. (1991) demonstrated that the PTGS1 gene maps to chromosome 9. Human prostaglandin endoperoxide synthase exhibited 91% aa identity with the sheep enzyme. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot (Human, mouse, rat): 0.1-0.5ug/ml
Immunohistochemistry (Paraffin) (Human): 0.5-1ug/ml. Antigen retrieval using heat is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
~0.5mg/ml (after reconstitution)
Synthetic peptide corresponding to aa35-52, CCYYPCQHQGICVRFGLD near the N-terminal of human COX1, different to the related rat and mouse sequence by 2aa.
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Recognizes human COX 1. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Yokoyama C. et al., Biochem Biophys. Res. Commun. 165:888-894 (1989) 2. Langenbach R. etal., Cell 83:483-492 (1995)