		Technical Data
C7904-75D	COX 1 (Cyclooxygenase 1, Cyclooxygenase-1, COX-1, COX1, Prostaglandin Endoperoxide Synthase-1, Prostaglandin G/H Synthase 1, PGH Synthase 1, PGHS1, PGHS-1, Prostaglandin G/H Synthase and Cyclooxygenase, Prostaglandin H Synthase 1, PHS 1, PHS1, PHS-1, Pros

Description:
Cyclooxygenase 1 (COX1), also known as Prostaglandin-endoperoxide synthase (PTGS1), is the key enzyme in prostaglandin biosynthesis. The gene was approximately 40kb long, with 11 protein-coding exons. There were 599aa residues with a calculated molecular mass of approximately 68kD. By analysis of a human/hamster somatic hybrid DNA panel, Funk et al. (1991) demonstrated that the PTGS1 gene maps to chromosome 9. Human prostaglandin endoperoxide synthase exhibited 91% aa identity with the sheep enzyme. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot (Human, mouse, rat): 0.1-0.5ug/ml
Immunohistochemistry (paraffin)(Human): 0.5-1ug/ml. Antigen retrieval using heat is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml (after reconstitution)

Immunogen:

Synthetic peptide corresponding to a sequence near the N-terminal, aa 35-52, CCYYPCQHQGICVRFGLD, of human COX1, different to the related rat and mouse sequence by 2aa.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.

Specificity:

Recognizes human COX 1. Species Cross-Reactivity: mouse, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Yokoyama C. et al., Biochem Biophys. Res. Commun. 165:888-894 (1989) 2. Langenbach R. etal., Cell 83:483-492 (1995)