Technical Data
C7904-80A
COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)
Description:
Cyclooxygenases-1 and 2 (COX-1 and COX-2) also known as prostaglandin endoperoxide H synthases-1 and 2 are integral membrane proteins which catalyze the first step of prostaglandins, thromboxanes and procastacyclin production from arachidonic acid. Both COX-1 and COX-2 catalyze a cyclooxygenase (bis-oxygenase) reaction in which arachidonate plus two molecules of O2 are converted to PGG2 (prostaglandin G2) and a peroxidase reaction in which PGG2 undergoes a two-electron reduction to PGH2. COX-1 and COX-2 proteins are encoded by separate genes located on different chromosomes and both enzymes are very similar in structure. They are homodimeric, heme containing, glycosylated proteins with two catalytic sites but they differ substantially in their pattern of expression and biology 1, 2. COX-1 is expressed constitutively in most tissues and at high levels in selected cells and tissues, including endothelium, monocytes, platelets, renal collecting tubules and seminal vesicles. COX-1 is thought to regulate a number of housekeeping activities involved in renal, gastrointestinal and platelet function. Inducible COX-2 is undetectable in most mammalian tissues but its expression can be induced rapidly (26h) in fibroblasts, endothelial cells, monocytes and ovarian follicles in response to growth factors, tumor promoters, hormones, bacterial endotoxin and cytokines. COX-2 is also expressed constitutively in specialized cell types or tissues where it plays specific functions in individual biological processes. These include reproduction, immunity, renal physiology, neurotransmissiom, bone resorption and pancreatic secretion 1, 2. The COX-1 and COX-2 enzymes are important pharmacologically as targets of nonsteroidal anti-inflammatory drugs (NSAIDs) including aspirin, ibuprofen and the new COX-2 inhibitors. Inhibition of cyclooxygenases with NSAIDs acutely reduces inflammation, pain and fever. Some methods to determine COX activity include measuring uptake of oxygen using an oxygraph, measuring the conversion of radioactive arachidonic acid, or measuring prostaglandins formed from PGH2 (such as determining PGH2 using immunoassays 3). These methods can be complex, time consuming and are prone to interferences.

Applications:
Western Blot: 1-5ug/ml
ELISA
FACS
Optimal dilutions to be determined by researcher.
TypeIsotypeCloneGrade
MabIgG1,k4E51Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
Immunogen:
Recombinant human cyclooxygenase-2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Detects a ~68-70kD protein on Western blots from human origin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Smith, W.L., Garavito, R.M. and DeWitt, D.L. (1996) J. Biol. Chem. 271: 33157-33160.
2. Smith, W.L., DeWitt, D.L. and Garavito, R.M. (2000) Annu. Rev. Biochem. 69: 145-182.
3. Kargman, S., Wong, E., Greig, G.M., Falgueyret, J.P., Cromlish, W., Ethier, D., Yergey,
J.A., Riendeau, D., Evans, J.F., Kennedy, B., Tagari, P., Francis, D.A. and O'Neill, G.P.
(1996) Biochem. Pharmacol. 52: 1113-1125.