Technical Data
C7904-82
COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)
Description:
Two isoforms of Prostaglandin H synthase are well characterized, namely COX1 and COX2. Both forms of COX proteins are membrane associated heme proteins containing cyclooxygenase and peroxidase activities. These enzymes are targets of NSAID (Non steroidal anti-inflammatory drugs) such as aspirin. COX2 is a 72kD protein having 60% homology to COX1. It is induced by cytokines and mitogens. Most likely, it plays a role in inflammatory diseases such as rheumatoid arthritis.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-10ug/ml using Chemiluminescence.
ELISA: 1:10,000-100,000
Optimal dilutions to be determined by researcher.

Control Peptide: C7904-81

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide 17aa corresponding to human COX2 (KLH coupled).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.1% sodium azide.
Specificity:
Recognizes Cyclooxygenase 2. Species crossreactivity: Human, mouse, sheep and bovine. Expected to crossreact with rat based on sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hla, T., Neilson, K., PNAS USA 89: 7384-7388 (1992).