Technical Data
C7904-82F
COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)
Description:
Cyclooxygenase catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H2. Recent discoveries of the induction of cyclooxygenase biosynthesis by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothesis that the inducible form of cyclooxygenase, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions.10 COX-2 is a 72kDa protein which has been cloned from a variety of species including human, mouse, rat, and sheep.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml ( 1:1000)
Immunohistochemistry: 1:50-1:100
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16D318Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa 580-599 of human COX-2. (NASSSRSGLDDINPTVLLKE).
Purity:
Purified.
Form
Supplied as a lyophilized powder.Reconstitution: Reconstitute with 100ul sterile, ddH2O or sterile 40-50% glycerol.
Specificity:
Recognizes human COX-2. Species Crossreactivity: monkey and sheep. Does not crossreact with mouse, rat or rabbit COX-2 or COX-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.