Cyclooxygenase catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H2. Recent discoveries of the induction of cyclooxygenase biosynthesis by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothesis that the inducible form of cyclooxygenase, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions. COX-2 is a 72 kDa protein which has been cloned from a variety of species including human, mouse, rat, and sheep.
Suitable for use in Flow Cytometry. Not recommended for Western Blot and Immunohistochemistry. Other applications not tested.
Flow Cytometry: Use 1-2ug antibody per 10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
|Synthetic peptide corresponding to aa 580-599 (NASSSRSGLDDINPTVLLKE) of human COX-2.|
|Supplied as a liquid in PBS, pH 7.2, 4mg/ml BSA, 0.02% sodium azide, 50% glycerol. Labeled with fluorescein isothiocyanate (FITC).|
|Recognizes human COX-2. Species crossreactivity: sheep. Species sequence homology: bovine and canine. Does not cross-react with mouse, rat, and rabbit COX-2 or COX-1 (all species). |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1.Hla, T., Neilson, K.. Human cyclooxygenase-2 cDNA. Proc Natl Acad Sci USA 89 7384-7388 (1992).2. Xie, W., Chipman, J.G., Robertson, D.L., et al. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc Natl Acad Sci USA 88 2692-2696 (1991).3. Kujubu, D.A., Fletcher, B.S., Varnum, B.C., et al. TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue. J Biol Chem 266 12866-12872 (1991).4. Yamagata, K., Andreasson, K.I., Kaufmann, W.E., et al. Expression of a mitogen-inducible cyclooxygenase in brain neurons: Regulation by synaptic activity and glucocorticoids. Neuron 11 371-386 (1993).5. Zhang, V., O'Sullivan, M., Hussain, H., et al. Molecular cloning, functional expression, and selective regulation of ovine prostaglandin H synthase-2. Biochem Biophys Res Commun 227 499-506 (1996).6. Litherland, S.A., Xie, X.T., Hutson, A.D., et al. Aberrant prostaglandin synthase 2 expression defines an antigen-presenting cell defect for insulin-dependent diabetes mellitus. J Clin Invest 104 515-523 (1999).|