Cyclooxygenase catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H2. Recent discoveries of the induction of cyclooxygenase biosynthesis by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothesis that the inducible form of cyclooxygenase, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions. COX-2 is a 72kD protein which has been cloned from a variety of species including human, mouse, rat, and sheep.
Suitable for use in Flow Cytometry and Immunocytochemistry. Not recommended for Western Blot and Immunohistochemistry. Other applications not tested.
Flow Cytometry: Use 1ug antibody per 10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
|Synthetic peptide corresponding to aa580-599, NASSSRSGLDDINPTVLLKE of human COX-2. Species sequence homology: bovine and canine.|
|Supplied as a liquid in PBS, pH 7.2, 4mg/ml BSA, 0.02% sodium azide, 50% glycerol. Labeled with fluorescein isothiocyanate (FITC).|
|Recognizes human COX-2. Species crossreactivity: sheep. Does not cross-react with mouse, rat, and rabbit COX-2 or COX-1 (all species). |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1.Hla, T., Neilson, K.. Human cyclooxygenase-2 cDNA. Proc Natl Acad Sci USA 89 7384-7388 (1992).2. Xie, W., Chipman, J.G., Robertson, D.L., et al. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc Natl Acad Sci USA 88 2692-2696 (1991).3. Kujubu, D.A., Fletcher, B.S., Varnum, B.C., et al. TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue. J Biol Chem 266 12866-12872 (1991).4. Yamagata, K., Andreasson, K.I., Kaufmann, W.E., et al. Expression of a mitogen-inducible cyclooxygenase in brain neurons: Regulation by synaptic activity and glucocorticoids. Neuron 11 371-386 (1993).5. Zhang, V., O'Sullivan, M., Hussain, H., et al. Molecular cloning, functional expression, and selective regulation of ovine prostaglandin H synthase-2. Biochem Biophys Res Commun 227 499-506 (1996).6. Litherland, S.A., Xie, X.T., Hutson, A.D., et al. Aberrant prostaglandin synthase 2 expression defines an antigen-presenting cell defect for insulin-dependent diabetes mellitus. J Clin Invest 104 515-523 (1999).|