Technical Data
COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)
COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin):1:50-1:100 for 10 minutes at RT. Boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes is required.
Immunohistochemistry: 1:10-1:1:500
Western Blot: 1:100-1:2000
Optimal dilutions to be determined by the researcher.

Positive Control:
Lung carcinoma and colon carcinoma

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
500ul4C Do Not FreezeBlue IceRatRabbit
Recombinant protein corresponding to the C-terminus of rat COX2. Cellular Localization: Microsomal membrane, cytoplasmic
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 10mM sodium azide.
Recognizes rat COX 2 at ~70kD. Species Crossreactivity: human and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.