Technical Data
C7904-83B
COX 2 (PTGS2, Prostaglandin-endoperoxide Synthase 2, Prostaglandin G/H Synthase and Cyclooxygenase, COX2, COX-2, PHS-2, PGG/HS, PGHS-2, hCox-2, Prostaglandin G/H Synthase and Cyclooxygenase)
Description:
Cyclooxygenase (Cox) is the key enzyme in conversion of arachidonic acid to PGs, and two isoforms, Cox-1 and Cox-2, have been identified. Cox-2 gene encodes an inducible prostaglandin synthase enzyme that is overexpressed in adenocarcinomas and other tumors. Deletion of the murine Cox-2 gene in Min mice reduced the incidence of intestinal tumors, suggesting that it is required for tumorigenesis. This gene is localized to sites associated with retinal blood vessels, and plays an important role in blood vessel formation in the retina. And the glucocorticoid receptor suppression of COX-2 is also crucial for curtailing lethal immune activation, and suggest new therapeutic approaches for regulation of T-cell-mediated inflammatory diseases.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 0.5-1ug/ml. Requires antigen retrieval using heat.
Western Blot: 0.1-0.5ug/ml
Optimum dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Synthetic peptide corresponding to a sequence at the N-terminal, aa26-41, CQNRGVCMSVGFDQYK, of human COX-2, different to the related rat sequence by 2aa.
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity:
Recognizes human COX 2. Species Cross-Reactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. SAlenkivi, K et al., J Clin Endocr Metab 86:5615-5619 (2001) 2. Liu et al., J. Biol. Chem. 276:18563-18569 (2001) 3.Wilkinson-Berka J.L., et al., Invest. Ophthal. Vis. sci. 44: 974-979 (2003) 4. Brewer J.A., etal., Nature Med. 9: 1318-1322 (2003)