Technical Data
COX 4-1 (COX IV-1)
Cytochrome C Oxidase (COX), which is located in the inner mitochondrial membrane, is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced Cytochrome C and donates them to molecular oxygen, which is then reduced to water.
Antibodies to this protein make an effective loading control for mitochondria. COXIV (COX4 )is generally expressed at a consistently high level. However, be aware that many proteins run at the same molecular weight as COXIV. Some caution is required when using antibodies to this protein as a loading control, as COXIV expression can vary under some manipulations.

Cellular Localization: Mitochondrion, mito inner membrane

Positive Control: HEK 293 lysates, Hela lysates

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ul-20CBlue IceHumanRabbit
A synthetic peptide made to an internal region of the human COX41 protein (within residues 1-100). [Swiss-Prot# P13073]. Homology: 100% human, 99% mouse and cow, 92% monkey, 90% rat, and 76% Zebrafish
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2.
This antibody is specific for COX41. Species Crossreactivity: This antibody reacts with the human COX41 protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.