Technical Data
CPI-17 (PKC Potentiated Inhibitor Protein-17)
PKC Potentiated Inhibitor Protein-17 (CPI-17) is a 17kD protein. Expressed in smooth muscles and neuronal cells, CPI-17 is a myosin phosphatase inhibitor protein. Phosphorylation of CPI-17 at Thr38 enhances its inhibitory effect by 100 fold. Once phosphorylated, CPI-17 inhibits PP1c and MLCP holoenzyme activity.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.2-1ug/ml. Detects CPI-17 in L6 lysates. L6 lystate was resolved by electrophoresis, transferred to nitrocellulose and probed with C7905-50. Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ug-20CBlue IcePorcineRabbit
Recombinant protein corresponding to full length porcine CPI-17.
Purified by Protein A affinity chromatography.
Supplied as a liquid in Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 10% glycerol
Recognizes porcine CPI-17 at ~22kD. Species Crossreactivity: human, mouse, rat, bovine and rabbit. Does not crossreact with yeast.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Boopathi, E. et al., (2012) Mol. Cell Biology doi: 10.1128/ MCB.00626-12. 1. Eto, M., et al., FEBS Lett. 410: 356360 (1997). 2. Eto, M., et al., J. Biol. Chem. 276: 29,07229,078 (2001). 3. Eto, M., et al., Neuron 36: 1-20 (2002). 4. Ohki, S., et al., J. Mol. Biol. 314: 839849 (2001). 5. Eto, M., et al., J. Biochem. 118: 1104-1107 (1995). 6. Eto, M., et al., FEBS Lett. 410: 356-360 (1997). 7. Senba, S., Eto, M. & Yazawa, M., J. Biochem. 125: 354-362 (1999).