Technical Data
CPI-17 (PKC Potentiated Inhibitor Protein-17)
PKC Potentiated Inhibitor Protein-17 (CPI-17) is a 17kD protein. Expressed in smooth muscles and neuronal cells, CPI-17 is a myosin phosphatase inhibitor protein. Phosphorylation of CPI-17 at Thr38 enhances its inhibitory effect by 100 fold. Once phosphorylated, CPI-17 inhibits PP1c and MLCP holoenzyme activity.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.2-1ug/ml. Detects CPI-17 in L6 lysates. L6 lystate was resolved by electrophoresis, transferred to nitrocellulose and probed with C7905-50. Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ug-20CBlue IcePorcineRabbit
Recombinant protein corresponding to full length porcine CPI-17.
Purified by Protein A affinity chromatography.
Supplied as a liquid in Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 10% glycerol
Recognizes porcine CPI-17 at ~22kD. Species Crossreactivity: human, mouse, rat, bovine and rabbit. Does not crossreact with yeast.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Boopathi, E. et al., (2012) Mol. Cell Biology doi: 10.1128/ MCB.00626-12. 1. Eto, M., et al., FEBS Lett. 410: 356360 (1997). 2. Eto, M., et al., J. Biol. Chem. 276: 29,07229,078 (2001). 3. Eto, M., et al., Neuron 36: 1-20 (2002). 4. Ohki, S., et al., J. Mol. Biol. 314: 839849 (2001). 5. Eto, M., et al., J. Biochem. 118: 1104-1107 (1995). 6. Eto, M., et al., FEBS Lett. 410: 356-360 (1997). 7. Senba, S., Eto, M. & Yazawa, M., J. Biochem. 125: 354-362 (1999).