Technical Data
C7906-06B3
CPT1B (isoform 1) Carnitine Palmitoyltransferase 1B (muscle)
Description:
The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:16,000
Immunohistochemistry: 3-5ug/ml; In paraffin embedded Human Heart shows staining of myocardial fibres in longitudinal section.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-DIADLFQVPKAYS, from the C Terminus of human CPT1B (NP_689452.1; NP_689451.1; NP_004368.1; NP_001138609.1; NP_001138607.1; NP_001138606.1; NP_001138608.1.)
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human CPT1B. This antibody is expected to recognise human isoforms a (NP_689452.1; NP_689451.1; NP_004368.1; NP_001138609.1; NP_001138607.1), isoform c (NP_001138606.1) and isoform d (NP_001138608.1).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Yamazaki N, Shinohara Y, Shima A, Yamanaka Y, Terada H. Isolation and characterization of cDNA and genomic clones encoding human muscle type carnitine palmitoyltransferase I.Biochim Biophys Acta. 1996 Jun 7;1307(2):157-61.