Technical Data
C7906-74C
CAMP (Cathelicidin Antimicrobial Peptide, 18kD Cationic Antimicrobial Protein, CAP-18, hCAP-18, FALL-39 Peptide Antibiotic, CAP18, FALL39, HSD26)
Description:
CAMP (Cathelicidin AntiMicrobial Peptide also18kD cationic antimicrobial protein, CAP18, LL37, FALL39 and HSD26) is a member of the cathelicidin family of proteins. It is widely expressed, being found associated with neutrophils, bronchial epithelium, renal tubule epithelium, activated keratinocytes, gd T cells, monocytes, NK cells, colonic epithelium and the stratum basale of nonkeratinized epithelium found in the vagina and oral cavity. CAMP has marked antimicrobial activity against both Gm+ and Gm- bacteria, and acts as a chemoattractant for neutrophils, monocytes and mast cells. CAMP is synthesized as 170aa preproprecursor. It contains a 30aa signal sequence, a 103aa, 14kD prosegment (aa31-131) and a 4-5kD, 37aa (aa134-170) C-terminal mature fragment (LL37) or 39aa (aa132-170) C-terminal mature fragment (FALL39). In neutrophils, the 18-19kD proprecursor is stored in granules, where, upon activation, it is enzymatically cleaved and released. While both the prosegment and C-terminal fragments possess antimicrobial activity, the prosegment also shows protease activity, while the C-terminal fragment also shows chemotactic activity. The prosegment may form homodimers, while the C-terminal fragment (LL37) is reported to form homotetramers. Over aa141-170, human CAMP shares only 50% and 57% aa sequence identity with mouse and rat CAMP, respectively.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanSheep
Concentration:
~0.1mg/ml after reconstitution
Immunogen:
Recombinant corresponding to aa134-170 from human CAMP expressed in E.coli.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity:
Recognizes human CAMP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.