Technical Data
C7906-75
Cranin (Dystroglycan)
Description:
Cranin is expressed in brain and many other tissues, it binds laminin with high affinity in a calcium dependent manner. It appears to be important in maintaining normal muscle integrity. Loss of cranin from the muscle surface may be one of the primary events leading to muscle injury in congenital muscular dystrophies, it may also play a role in the cognitive deficits often seen in such conditions.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Not recommended for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:1-1:50 detects Cranin on tissue fixed briefly with 0.5% glutaraldehyde, 4% paraformaldehyde, methanol or acetone. Prolonged fixation must be avoided.
Immunocytochemistry: Neuron, astrocyte, smooth muscle, fibroblast and epithelial cell lines.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM2Q36Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to aa572-604 of human Cranin.
Purity:
Ascites
Form
Supplied as a liquid.
Specificity:
Recognizes human Cranin (dystroglycan), extracellular site, near the C-terminus of the alpha-subunit. The expression and localization of cranin varies considerably according to the cells physiologic state and differentiaion state. Species Crossreactivity: rat, mouse, sheep, chicken and Xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. PNAS USA 84: 64576461 (1987). 2. J. Neurosci. Res. 36: 528538 (1993). 3. J. Biol. Chem. 270: 15,42515,433 (1995).