Technical Data
C7907-04N
C-Reactive Protein (CRP, MGC88244, PTX 1, PTX1)
Description:
C-reactive protein (CRP) is a calcium-binding acute phase reactant belonging to the pentraxin family which is secreted into the plasma by the liver, in response to cytokine stimulation. In addition to binding to phosphocholine residues, CRP can bind to other autologous and extrinsic ligands on targets, including damaged cell membranes, ribonucleoprotein particles, apoptotic cells and constituents of micro-organisms, and may activate the classical complement pathway through C1q recognition, making CRP a factor in a variety of host defense mechanisms. Levels of plasma CRP are significantly increased during the acute phase response, tissue injury and infection and can be used diagnostically as a marker of inflammation, disease progress and risk assessment of cardiovascular disease (CVD).

Applications:
Suitable for use in ELISA, Western Blot, Immunoaffinity purification and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: Direct
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG19B50Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human CRP, derived from pleural/ascetic fluid or plasma.
Purity:
Purified by Protein A chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the human CRP antigen both in the presence and in the absence (samples containing EDTA) of Ca2+.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.