Technical Data
C7915-05C
CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein)
Description:
The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMPdependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:2000. Detects bands of 46 and 43kD corresponding to phosphorylated CREB.
Immunohistochemistry (FFPE): 20ug/ml
ELISA: 1:1000-1:6000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~0.05mg/ml
Immunogen:
Synthetic peptide corresponding to aa122-147, QKRREILSRRPpSYRKILNDLSSDAP, from human CREB Phosphorylated at pS133 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity:
Recognizes phosphorylated human CREB at pS133. Species Crossreactivity: mouse and rat. Minimal reactivity with non-phosphorylated CREB by Western Blot and minimal reactivity (1%) by ELISA against the non-phosphorylated form of the immunizing peptide. Assayed against a variety of tisues including fibroblasts and B-cell lysates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Moreno, C.S., et al . (1999) CREB regulates MHC class II expression in a CIITA dependent manner. Immunity 10: 143-151.. (2000) MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells. FEBS Lett, 482(1-2): 44-8.2. Lee CQ, Yun YD, Hoeffler JP, Habener JF. (1990) EMBO J. 9(13):4455-4465. 3. Dwarki VJ, Montminy M, and Verma IM. (1990)EMBO J., 9(1):225-232 3.Kwok RP, Laurance ME, Lundblad JR, Goldman PS, Shih H, Connor LM, Marriott SJ, Goodman RH. (1996) Control of cAMP-regulated enhancers by the viral transactivator Tax through CREB and the co-activator CBP. Nature 380:642-646. 5. Barton K, Muthusamy N, Chanyangam M, Fischer C, Clendenin C, Leiden JM. (1996). Nature 379:81-85.Kim J., Lu J. and Quinn PG., (2000) Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation. Proc Natl Acad Sci U S A 97(21):11292-11296.