Technical Data
C7942-10
Cryptochrome (CRY)
Description:
Circadian rhythm is one of the most fascinating and complex biological phenomenon's. The circadian clock controls biological activities on daily light-dark cycles in species from cyanobacteria to humans. The circadian clock has three major components: A photoactive pigment (chromophore) for sensing light and transmitting light signals, the circadian clock that oscillates every ~24-hrs, and the genes controlled by the circadian clock to bring about the physiological and behavioral changes. Several genes (Drosophila Clock Per, Tim; mammalian Per1, Per2, Per3, MOP3 and MOP4, and BMAL1) have been linked to rhythmicity or circadian behavior of living organisms. However, very little is known about the mammalian photosensory molecules.

Most recently mammalian homolog of the plant blue-light photoreceptors termed cryptochromes have been identified. Mouse CRY1 and CRY2, are 606 aa and 569 aa protein, respectively. Cry1 and Cry are specifically expressed in ganglion cell and inner nuclear layers of the mouse retina. CRY1 is expressed at high level in the SCN and oscillates in a circadian manner. Like other genes implicated in circadian mechanism, CRY1 and CRY2 are also expressed in most animal tissues. Therefore, mammalian cryptochromes appears to play an important role in entrainment of the circadian clock.

Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.

ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceDrosophilaRabbit
Concentration:
As reported
Immunogen:
An 18 aa peptide sequence within the C-terminus of Drosophila CRY (1) was synthesized coupled to KLH
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
Specificity:
The mouse peptide sequence is 92% conserved in flesh fly and 76% in Chinese silk moth cryptochrome. No significant homology is seen with mammalian CRY1/2. Antibody crossreactivity with CRY from various species is not known. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommed for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of aff pure IgG or 1 ul of antiserum) to demonstrate antibody specificity
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Emery P et al (1998) Cell 95, 669-679; Stanewsky R et al (1998) Cell 95, 681-692; Miyamoto Y and Sancar A (1998) 95, 6097-6102