		Technical Data
C7944-60C	Crystallin AB (Alpha crystallin B chain, CryAB, Alpha (B)-crystallin, Rosenthal fiber component, Heat-shock protein beta-5, HspB5, CryA2, AACRYA, Alpha crystallin B chain, CRYA2, Crystallin alpha polypeptide 2, CTPP 2, CTPP2, Heat shock 20kD like protein,

Description:
AlphaB Crystallin (CRYAB, also known as Crystallin alpha-B chain and HspB5) is a 22-23kD member of the HSP20 family of proteins. It has widespread expression in many tissues in addition to lens epithelium, where it noncovalently oligomerizes with CRY-alphaA to generate a transparent 350-1000kD alpha-Crystallin protein complex. Human CRYAB is 175aa in length. There is a alpha-Crystallin Hsp domain over aa66-149. Multiple posttranslational modifications may exist. The N-terminal Met and MetAspIleAlaIleHis sequence is occasionally cleaved. There is also phosphorylation at Ser45 and 59, potential O-GlcNAc modification at Thr158, 162 or 170, and acetylation at Lys92. There is an alternate start site at Met68 that may be accompanied by a 47aa substitution for aa109-175. Full-length human CRYAB is 54% aa identical to CRY-AlphaA, and 98% aa identical to mouse CRYAB.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.1ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-28C: IgG, H&L (HRP) Pab DkxGt
I1904-28G: IgG, H&L (HRP) (X-Adsorbed) Pab DkxGt
I1904-28K: IgG, H&L (HRP) Pab RbxGt
I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~1mg/ml

Immunogen:

E. coli-derived recombinant human Crystallin AB (aa1-175, Accession #P02511)

Purity:

Purified by affinity chromatography.

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 100ul sterile PBS, 0.02% sodium azide.

Specificity:

Recognizes human Crystallin AB (CRYAB) at ~23kD. Does not crossreact with recombinant human CRYAA in Western Blots. Species Crossreactivity: mouse and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.