Technical Data
CRYAB (Alpha-crystallin B Chain, Alpha(B)-crystallin, Heat Shock Protein beta-5, HspB5, Renal Carcinoma Antigen NY-REN-27, Rosenthal Fiber Component, CRYA2)
a-b-Crystalline is a lens protein, which has some homology with the small heat shock proteins. It is expressed in tissues such as skeletal muscle, cardiac muscle, smooth muscle, renal tubular epithelium, Schwanncells, glial cells, thyroid epithelium, colonic epithelium and stratified squamous epithelium. This antibody may be useful in the identification of Lewy body dementia.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 02.5-5ug/ml
Immunohistochemistry: 1:200 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at room temperature for 20min
Optimal dilutions to be determined by the researcher.

Positive Control:

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabAffinity Purified
50ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to a protein sequence close to the N-terminus of human alpha-B-crystalline.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Recognizes human alpha-B-crystalline.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.