		Technical Data
C7944-72C	Crystallin, alpha A (alpha A Crystallin Protein, Alpha Crystallin A Chain, CRYAA, Acry-1, Crystallin Alpha 1, CRYA1, Heat Shock Protein beta 4, HSPB4)

Description:
Besides the involvement of both alpha A and alpha B crystallin in the prevention of protein precipitation and cataract formation in the eye, alpha crystallins have also been demonstrated in other tissues including the heart, brain, kidney, spleen and thymus.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1,000-1:2,000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	10B2610	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant human alpha A crystallin

Purity:

Purified by protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 10% glycerol.

Specificity:

Recognizes human alpha A crystallin, a 20kDa water-soluble major structural protein and member of the heat shock protein family, designated HSPB4, which acts as a molecular chaperone and is believed to play a role in the maintenance of ocular lens transparency/refractive index. Species crossreactivity: rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Andley, U. P. et al. (1996) Cloning, expression, and chaperone-like activity of human alphaA-crystallin. J. Biol. Chem. 271: 31973-31980.