Technical Data
C7944-75J
Crystallin, alpha B, phosphorylated (Ser45)
Description:
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 dImmunofluorescenceferent proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identImmunofluorescenceied in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander’s disease where it accumulates in brain cells of those afflicted.

Application(s):
Suitable for use in Immunofluorescence, Immunohistochemistry (parafin embedded) and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 5ug/ml; Immunofluorescence staining of phospho-alpha-B crystallin (Ser45) in U373 MG cells results in cytoplasmic staining.
Immunohistochemistry (parafin embedded): 8ug/ml
Western Blot: 0.5ug/ml; Detects a band of ~20kD representing recombinant bovine phospho-alpha-B crystallin (Ser45).

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide: LS pPFYLRPPSF, corresponding to amino acids 44-54 of Human Crystallin (alpha B).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, 30mg/ml BSA, 0.05% sodium azide.
Specificity:
Recognizes Crystallin, alpha B, when phosphorylated at Ser45.Does not detect the unphosphorylated form of the protein. Species Crossreactivity: Bovine, Human, Mouse and Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.