Technical Data
Crystallin, alpha B, phosphorylated (Ser45)
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 dImmunofluorescenceferent proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identImmunofluorescenceied in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander’s disease where it accumulates in brain cells of those afflicted.

Suitable for use in Immunofluorescence, Immunohistochemistry (parafin embedded) and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 5ug/ml; Immunofluorescence staining of phospho-alpha-B crystallin (Ser45) in U373 MG cells results in cytoplasmic staining.
Immunohistochemistry (parafin embedded): 8ug/ml
Western Blot: 0.5ug/ml; Detects a band of ~20kD representing recombinant bovine phospho-alpha-B crystallin (Ser45).

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20°CBlue IceHumanRabbit
As reported
Synthetic peptide: LS pPFYLRPPSF, corresponding to amino acids 44-54 of Human Crystallin (alpha B).
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder in PBS, 30mg/ml BSA, 0.05% sodium azide.
Recognizes Crystallin, alpha B, when phosphorylated at Ser45.Does not detect the unphosphorylated form of the protein. Species Crossreactivity: Bovine, Human, Mouse and Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.