Technical Data
Crystallin, alpha B, phosphorylated (Ser59)
Alpha-crystallins composed of alpha-A (~19kD) and alpha-B (~19.2kD) subunits, are major water-soluble proteins accounting for almost 50% of the total protein of the mammalian transparent eye lens and they are also found in a variety of other tissues. The alpha-crystallins have sequence homology as well as structural and functional similarities with the small heat shock proteins such as Hsp25. The alpha-crystallins and Hsp25 have been shown to act as molecular chaperones by suppression of heat-induced aggregation of other proteins and are efficient inhibitors of elastase. The alpha-B subunit like Hsp25 was also found to be a substrate for protein cross-linking by tissue-type transglutaminase. The alpha-crystallins may be involved in metabolic pathways important for the development, maintenance, or pathology of the lens. They prevent destabilized proteins from precipitating out of solution by forming a soluble high-molecular-weight (HMW) complex. The bovine HMW protein form of the lens is composed mostly of alpha-crystallin in a highly aggregated state which has markedly reduced chaperone ability compared to alpha-crystallin. NMR spectroscopy of the naturally occurring HMW complex shows that the short C-terminal extension of alpha-B subunit in HMW has lost its flexibility whereas the alpha-A subunit extension is still flexible. These results suggest that depletion of alpha- crystallin with age as it is converted to HMW, could be one of the contributing factors to cataract formation in the lens.

Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml
Immunohistochemistry (Paraffin): 8ug/ml
Immunocytochemistry: 8ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Not Determined
Synthetic peptide corresponding to aa54-64,FLRAPpSWIDTG of human alpha B-crystallin phosphorylated at Ser59.
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, pH 7.2, 30mg/ml BSA, 0.05% sodium azide. Reconstitute with sterile ddH2O.
Recognizes human alpha B-crystallin when phosphorylated at Ser59 at ~20kD by Western Blot. Does not cross-react with the unphosphorylated protein. Species Crossreactivity: rat and bovine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.