Technical Data
C7944-75P
Crystallin, alpha B, phosphorylated (Ser59)
Description:
Alpha-crystallins composed of alpha-A (~19kD) and alpha-B (~19.2kD) subunits, are major water-soluble proteins accounting for almost 50% of the total protein of the mammalian transparent eye lens and they are also found in a variety of other tissues. The alpha-crystallins have sequence homology as well as structural and functional similarities with the small heat shock proteins such as Hsp25. The alpha-crystallins and Hsp25 have been shown to act as molecular chaperones by suppression of heat-induced aggregation of other proteins and are efficient inhibitors of elastase. The alpha-B subunit like Hsp25 was also found to be a substrate for protein cross-linking by tissue-type transglutaminase. The alpha-crystallins may be involved in metabolic pathways important for the development, maintenance, or pathology of the lens. They prevent destabilized proteins from precipitating out of solution by forming a soluble high-molecular-weight (HMW) complex. The bovine HMW protein form of the lens is composed mostly of alpha-crystallin in a highly aggregated state which has markedly reduced chaperone ability compared to alpha-crystallin. NMR spectroscopy of the naturally occurring HMW complex shows that the short C-terminal extension of alpha-B subunit in HMW has lost its flexibility whereas the alpha-A subunit extension is still flexible. These results suggest that depletion of alpha- crystallin with age as it is converted to HMW, could be one of the contributing factors to cataract formation in the lens.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml
Immunohistochemistry (Paraffin): 8ug/ml
Immunocytochemistry: 8ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to aa54-64,FLRAPpSWIDTG of human alpha B-crystallin phosphorylated at Ser59.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 30mg/ml BSA, 0.05% sodium azide. Reconstitute with sterile ddH2O.
Specificity:
Recognizes human alpha B-crystallin when phosphorylated at Ser59 at ~20kD by Western Blot. Does not cross-react with the unphosphorylated protein. Species Crossreactivity: rat and bovine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.