Technical Data
Crystallin, alpha B
Alpha-Crystallins are composed of two gene products; alpha-Crystallin A and B, for acidic and basic, respectively. Alpha-Crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-Crystallin A and B subunits have a 3:1 ratio, respectively. Two additional functions of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. alpha-Crystallin B is expressed widely in many tissues and organs and occurs in many neurological diseases.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.

Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2b,k7G26Affinity Purified
50ul-20CBlue IceHumanMouse
Recombinant human alpha-Crystallin B (1-175aa) (E. coli)
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.01% sodium azide.
Recognizes human Alpha-Crystallin B
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.