Technical Data
C7944-75T
Crystallin, alpha B
Description:
Alpha B Crystallin, first identified as a structural component of the vertebrate eye lens, is expressed at high levels in lens and at lower levels in a number of other tissues, most notably cardiac and skeletal muscle, kidney, and brain (1). It is a major protein component of Rosenthal fibers, which massively accumulate in the brains of patients suffering from Alexander's disease (2). The major phosphorylation site of Alpha B Crystallin in brains of patients with Alexander's disease or Alzheimer's disease as well as in aged controls is Serine 59 (3). Racemization, isomerization, and oxidation of Alpha B Crystallin are said to occur spontaneously in the aging process (4).

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5,000-50,000
Immunohistochemistry: 1:100-250
Immunocytochemistry: 1:25-50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E234Serum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues on the C terminus of human Alpha B Crystallin.
Purity:
Serum
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Specificity:
Recognizes human Crystallin, alpha B.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dubin RA, et al. Genomics 7(4): 594-601, 1990. 2. Iwaki A, et al. Neurosci Lett 140(1): 89-92, 1992
3. Kato K, et al. J Neurochem 76(3): 730-6, 2001. 4. Fujii N, et al. Biochim Biophys Acta 1204(2): 157-63, 1994