Technical Data
C7944-75T
Crystallin, alpha B
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Technical Data |
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C7944-75T |
Crystallin, alpha B |
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Description: Alpha B Crystallin, first identified as a structural component of the vertebrate eye lens, is expressed at high levels in lens and at lower levels in a number of other tissues, most notably cardiac and skeletal muscle, kidney, and brain (1). It is a major protein component of Rosenthal fibers, which massively accumulate in the brains of patients suffering from Alexander's disease (2). The major phosphorylation site of Alpha B Crystallin in brains of patients with Alexander's disease or Alzheimer's disease as well as in aged controls is Serine 59 (3). Racemization, isomerization, and oxidation of Alpha B Crystallin are said to occur spontaneously in the aging process (4). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5,000-50,000 Immunohistochemistry: 1:100-250 Immunocytochemistry: 1:25-50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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