		Technical Data
C7944-93	Crystallin, mu (mu Crystallin, CRYM, DFNA40, NADP-regulated Thyroid Hormone Binding Protein, THBP) (Azide Free)

Description:
Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1.5ug/ml
ELISA: 1ug/ml-3ng/ml
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	10B2609	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Recombinant protein corresponding to a portion of amino acids within aa215-315 of human CRYM.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).

Specificity:

Recognizes human CRYM.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Reed, P. W. et al (2007) Abnormal expression of mu-crystallin in facioscapulohumeral muscular dystrophy. Exp Neurol. 205: 583-6.