Technical Data
C7948-15H
11-CTP (Biotin) (g-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-cytidine-5-triphosphate, Triethylammonium Salt)
60ul
Molecular Biology Storage: -20CShipping: Blue Ice
11-CTP (Biotin) (Biotin-11-cytidine-5-triphosphate) can replace CTP for in vitro transcription reaction catalyzed by T3, T7 or SP6 RNA polymerases. The biotin-labeled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridization, sequencing, and genome analysis. The transcription reaction produces multiple RNA copies of the DNA template(s) during a short incubation period. RNA probes offer higher target specificity and greater sensitivity than the corresponding DNA-DNA hybrids.

Applications:
Suitable for use in Microarray based hybridization detection and Ribonuclease protection assay
Other applications have not been tested.

Spectroscopic Properties:
max 294 nm; 9300

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Formula:
C28H44N7O17P3S

Molecular Weight:
875.67
Purity: ~95%
Concentration: ~5mM
Form: Supplied as a liquid in buffered solution, pH 7.5. Labeled with Biotin.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Li, et al., Physiol. Genomics 27: 42 (2006). 2. Jelinsky, et al., Proc. Natl. Acad. Sci. USA 96: 1486 (1999). 3. Kenrick, et al., A homogeneous method to quantify mRNA levels: a hybridization of Rnase protection and scintillation proximity assay technologies. Oxford University Press: 2947 (1995).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.