Technical Data
C7948-50
CUG-Binding Protein 1 Ribonucleoprotein (CUG-BPI, Cytidine Uridine Guanosine Binding Protein 1)
Description:
Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Supershift Assay. Other applications have not been tested.

Recommended Dilution:
Western Blot Analysis: 0.1-2.0ug/ml detects CUG-BP1 in HeLa nuclear extract. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with C7948-50 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: Immunoprecipitates CUG-BP1 from HeLa cell lysates
Immunocytochemistry: Nuclear staining in HeLa and myoblasts from normal patients.
Supershift: Supershifts CUG-BP1:DNA complexes in EMSA
Optimal dilutions to be determined by the researcher.

Recommended secondary antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(ab)2 (HRP) Pab Gt xMo

Included Positive Antigen Control:
H1838: HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1.T.9Affinity Purified
SizeStorageShippingSourceHost
200ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Full-length GST fusion protein corresponding to human CUG-BP1, also known as heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.07% sodium azide, before the addition of glycerol to 30%
Specificity:
Recognizes human CUG-BP1, Mr 50kD. Species Crossreactivity: Reported to crossreact with mouse, rat, bovine, porcine and rabbit
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Timchenko LT, et al. 1996. Nucleic Acids Res. 24 (22): 44074414.