Technical Data
C7948-50F
CUGBP2 (CUGBP Elav-like Family Member 2, CELF-2, Bruno-like Protein 3, CUG Triplet Repeat RNA-binding Protein 2, CUG-BP2, CUG-BP- and ETR-3-like Factor 2, ELAV-type RNA-binding Protein 3, ETR-3, Neuroblastoma Apoptosis-related RNA-binding Protein, hNAPOR,
Description:
RNA editing is an important mechanism for regulating genetic plasticity through the generation of alternative protein products from a single structural gene. There are two types of substitutional RNA exist in mammals, namely A-to-I and C-to-U RNA editing. The best-characterized example of C-to-U RNA editing involves the nuclear transcript encoding intestinal apolipoprotein B (apo B)). Apo B RNA editing changes a CAA to a UAA stop codon, generating a truncated protein, apoB48. The functional complex includes a minimal core composed of apobec-1 and ACF, that function as an adaptor protein by binding both the deaminase and the RNA substrate. The RNA binding proteins also include CUGBP2 which along with Apobec-1 binds to the consensus binding sequence UUUN (A/U) U, present in c-myc, VEGF and Cyclooxygenase-2 (COX2).

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml (ECL)
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to 18aa of human CUGBP2 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity:
Recognizes human CUGBP2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shrikant Anant et al (2001) JBC, Vol. 276, No: 50, 47338-47351. 2. Duanxiang Li et al (2001) Genomics 74, 396-401. 3. Lu X et al (1999) Hum. Mol. Genet. 8 (1), 53-60.