Technical Data
C7949-01
Cullin 1 (CUL-1)
Description:
In yeast, proteolysis of G1 cell cycle regulatory proteins is controlled by a ubiquitin ligase formed by three subunits: Cdc53 (also known as CulA), Skp1 and one of many F-box proteins (reviewed in ref 2). A C. elegans family of cullin genes homologous to the S. cerevisić cdc53 gene has been identified (cul1, 2, 3, 4, and 5) (2). The C. elegans cul1 null mutant shows a shortened G1 phase and a generalized hyperplasia, suggesting that Cul1 may be involved in the degradation of positive cell cycle regulators (e.g., G1 cyclins as in S. cerevisić). These findings suggest that during evolution the cdc53 gene diverged into at least five different genes, of which some may have specialized in the ubiquitination of specific proteins (e.g., cyclins) and others in the ubiquitination of other proteins (e.g., Cdk-inhibitors). A family of 6 human cullin genes (cul1,2, 3, 4a, 4b, and 5) has also been identified. It was then shown that Cul1 forms a complex with human Skp1 and the F box protein Skp2. This complex is thought to play a role in the ubiquitination of G1 regulatory proteins as its homolog does in yeast.

Applications:
Suitable for use in Immunoprecipation, Western Blot and ELISA. Other applications have not been tested.

Recommended Dilutions:
ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunoprecipitation (Native): 5-10ug/ IP reaction
Optimal dilutions to be determined by the researcher.

Positive Control:
IMR90 extracts (human fibroblasts) and Wi-38 cells.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG1,k6A56Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to an 11aa sequence at the C-terminus of the human Cullin 1.
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human Cullin 1 at ~ 86kD. Does not crossreact with the related CUL-2 protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kipreos, E.T., et al; Cell 85: 829-839 (199). 2. Pagano, M., FASEB J. 11:1067-1075 (1997).