Technical Data
C7949-01F
Cullin 1 (CUL-1)
Description:
Cullin proteins assemble a large number of RING E3 ubiquitin ligases and regulate various physiological processes (1). It has been shown that the SKP1, CUL1, F-box (SCF) containing pathway, although similarly used by the mammalian CUL1, is not shared by other cullin members. This implies that most cullins may use a SKP1/F-box-independent pathway to facilitate protein degradation (2). The majority of CUL1 has been found in a complex with CAND1 and ROC1, independent of SKP1 and F-box protein SKP2. Data suggests that CAND1 regulates the formation of the SCF complex, and its dissociation from CUL1 is coupled with the incorporation of F-box proteins into the SCF complex, causing their destabilization (3).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:10,000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E237Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic peptide corresponding to residues on the C-terminus of human CUL1.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human Cullin 1 (CUL-1). Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Liu J, et al. Mol Cell. 10(6):1511-8, 2002. 2. Michel JJ, et al. Cell Growth Differ. 9(6):435-49, 1998 3. Zheng J, et al. Mol Cell. 10(6):1519-26, 2002