Technical Data
C7949-10D
Cullin 2 (CUL -)
Description:
Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 2 (CUL 2) is a component of E3 ubiquitin ligase complexes which controls the proteolysis of G1 cell cycle regulatory proteins. It forms a complex with an inactive transcriptional elongation complex formed by three subunits elongain C, elongain B and VHL.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:100
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide (RSQASADEYSYVA) corresponding to aa733-745 of human Cullin 2
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes human CUL 2
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Michel, J.J. & Xiong, Y. (1998) Cell Growth Differ. 9,435-449. Liakopoulos, D. et al. (1999) Proc. Natl. Acad. Sci. USA 96,5510-5515.