Technical Data
Cullin 2 (CT) (CUL-2)
In yeast, proteolysis of G1 cell cycle regulatory proteins is controlled by a ubiquitin ligase formed by three subunits: Cdc53 (also known as CulA), Skp1 and one of many F-box proteins. A family of human cullin genes homologous to the S. cerevisiae cdc53 gene has been identi ed (cul1, 2, 3, 4a, 4b, and 5) . It was then shown that Cul1 forms a complex with human Skp1 and the F box protein Skp2. In contrast, Cul2 does not associate with Skp1 and Skp2. Instead it forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongin C, Elongin B and VHL. The meaning of this association is still unknown. Intriguingly, Elongin C shows homology with Skp1 and Elongin B with ubiquitin. In addition, in its active form, SIII contains, instead of VHL, an F box protein: Elongin A.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:2,000-1:10,000
Western Blot: 1:500-1:1,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide, RSQASADEYSYVA, corresponding to aa733-745 of human Cul2, C-terminus (KLH).
Supplied as a liquid, 0.01% sodium azide.
Recognizes human Cullin 2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Feng, H., et al., (1999), "CUL-2 is required for the G1-to S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans", Nat. Cell Bio., 1, 486-492. 2. Kipreos, E. T., et al., (1996), "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family", Cell, 85 (6), 829-839. 3. Michel, J.J., et al., (1998), "Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A", Cell Growth Differ., 9 (6), 435-449. 4. Ohta, T., et al., (1999), "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity", Mol. Cell., 3 (4), 535-541. 5. Mathias, N., et al., (1996), "Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of protein", Mol. Cell Biol., 16:12, 6634-6643.