Technical Data
C7949-20B
Cullin 3 (CUL-3)
Description:
Involved in ubiquitination of cyclin E and, in complex with RBX1, of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Seems not be part of a SCF complex with SKP1. In complex with SPOP, involved in ubiquitination of both BMI1/PCGF4 and H2AFY.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Dot Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.5-2ug/ml
Western Blot: 0.2-2ug/ml
Immunocytochemistry: 2-100ug/ml
Immunoprecipitation: 100-500ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG18C90Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Partial recombinant sequence corresponding to human Cullin 3
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Specificity:
Recognizes human Cullin 3. Species Crossreactivity: mouse, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.