Technical Data
Cullin 3 (CUL-3)
Two distinct members of the Cullin gene family- Cul3 and Cul11- have been implicated in the ubiquitin-mediated destruction of mammalian cyclin E. Cul3 may be a core component of the E3 ubiquitin ligase complex that targets free cyclin E for destruction. Cul3 physically associates with cyclin E in mammalian cells, as would be expected if Cul3 was a component of the cyclin E ubiquitin ligase. Overexpression of Cul3 stimulates the accumulation of cyclin E-ubiquitin conjugates in a phosphorylation-independent manner, thereby demonstrating a critical link between Cul3 and the cellular cyclin E destruction machinery. Cul3 is likely to be required for destruction of cyclin E. However, it seems unlikely that the development defects are solely due to cyclin E accumulation, as Cul3 complexes will likely regulate the levels of other proteins in addition to cyclin E. Human Cul3 is a member of the Cullin family of E3 ubiquitin-protein ligases. Cul3 was found to bind to cyclin E but not to cyclin E-cdk2 complexes in mammalian cells, and that overexpression of Cul3 increased ubiquitination of cyclin E but not other cyclins. The SCF pathway, which targets cyclin for ubiquitination on the basis of their phosphorylation state, and the Cul3 pathway, which selects cyclin E for ubiquitination on the basis of its assembly into cdk complexes, may be a complementary way to control cyclin abundance.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 5-10ug/per IP reaction
Western Blot: 1-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Synthetic peptide derived from the N-terminal sequence of human Cullin 3
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Reacts with the ~89kD human Cullin 3 protein. Reactivity has been con rmed with HeLa, NIH 3T3 and 293T cells. Species sequence homology: mouse Cullin 3. Does not cross-react with other species
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Winston, J., et al., Genes and Dev. 13: 2,751-2,757, 1999. 2. Singer, J., et al., Genes and Dev. 13: 2,375-2,387, 1999.