Technical Data
C7949-21C
Cullin 3, NT (CUL3, Cullin-3, CUL-3, KIAA0617)
Description:
Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3. Component of an ubiquitin-protein E3 ligase complex which includes at least CUL3 and KLHL12. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000-1:10,000
Western Blot: 1:500-1:1000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to aa1-12 of human Cullin 3 at N-terminal (KLH).
Purity:
Serum
Form
Supplied as a liquid, 0.01% sodium azide.
Specificity:
Recognizes human Cullin 3. Species sequence homology: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.