Technical Data
C7949-21N
Cullin 5 (CUL-5)
Description:
Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 5 (CUL 5) is a component of E3 ubiquitin ligase complexes and it is involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kD protein. It may also form a cell surface vasopresin receptor and it may play a role in brast tumorigenesis.

Applications:
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:500
Western Blot: 1:1000
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide (SNLLKNKGSLQFEDK) corresponding to aa4-19 of human Cullin 5
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes human CUL 5
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Byrd, P.J. et al. (1997) Genome Res. 7,71-75. Fay, M.J. et al. (2003) Mol. Cancer 2,40,