		Technical Data
C7949-21N2	CUL5, CT (VACM-1, Vasopressin-activated calcium-mobilizing receptor, Cullin-5, CUL-5, CUL5, VACM1)

Description:
CUL5 is a core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAk2. It seems to be involved poteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55kD protein and may form a cell surface vasopressin receptor.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the C-terminal region of human CUL5 (KLH).

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human CUL5.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Kamura T., Burian D., Yan Q.J. Biol. Chem. 276:29748-29753(2001). 2.Mehle A., Goncalves J., Santa-Marta M.Genes Dev. 18:2861-2866(2004). 3.Kamura T., Maenaka K., Kotoshiba S.Genes Dev. 18:3055-3065(2004).