Technical Data
C8350-08
CXCR4 (Fusin, LESTR, HUMSTR, C-X-C Chemokine Receptor 4, CD184)
Description:
CXCR4, also termed Fusin, LESTR, or HUMSTR is a member of the G-protein-coupled chemokine receptor family with seven membranespanning domains and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CXCR4 is expressed predominantly on nave T cell subset of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. It is also expressed on neutrophils, monocytes and various cell lines.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Functional Application. Other applications not tested.

Recommended Dilution:
Immunocytochemistry (Acetone fixed cells): 1:300
Immunohistochemistry (Paraffin sections): 1:200
Western Blot: 1:1000
Functional Application: Blocks SDF mediated stimulation of DU-145 cells
Optimal dilutions to be determined by the researcher.

Recommended Positive Controls:
Human peripheral blood cells (Immunocytochemistry)
Human spleen cells (Paraffin) (Immunohistochemistry)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanGoat
Concentration:
~2mg/ml
Immunogen:
Synthetic peptide corresponding to aa 13-38 (CT[13]EEMGSGDYDSMKEPCFREENANFNK[38]) of the N-terminal extracellular domain of human CXCR4 (CXC chemokine receptor 4).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide.
Specificity:
Recognizes human CXCR4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes: M. Loetscher, et al.; J. Biol. Chem. 269: 232 (1994). 2. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry: C.C. Bleul, et al.; Nature 382: 829 (1996)1. Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes: M. Loetscher, et al.; J. Biol. Chem. 269: 232 (1994). 2. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry: C.C. Bleul, et al.; Nature 382: 829 (1996)