		Technical Data
C8350-08	CXCR4 (Fusin, LESTR, HUMSTR, C-X-C Chemokine Receptor 4, CD184)

Description:
CXCR4, also termed Fusin, LESTR, or HUMSTR is a member of the G-protein-coupled chemokine receptor family with seven membranespanning domains and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CXCR4 is expressed predominantly on naïve T cell subset of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. It is also expressed on neutrophils, monocytes and various cell lines.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Functional Application. Other applications not tested.

Recommended Dilution:
Immunocytochemistry (Acetone fixed cells): 1:300
Immunohistochemistry (Paraffin sections): 1:200
Western Blot: 1:1000
Functional Application: Blocks SDF mediated stimulation of DU-145 cells
Optimal dilutions to be determined by the researcher.

Recommended Positive Controls:
Human peripheral blood cells (Immunocytochemistry)
Human spleen cells (Paraffin) (Immunohistochemistry)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Human	Goat

Concentration:

~2mg/ml

Immunogen:

Synthetic peptide corresponding to aa 13-38 (CT[13]EEMGSGDYDSMKEPCFREENANFNK[38]) of the N-terminal extracellular domain of human CXCR4 (CXC chemokine receptor 4).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide.

Specificity:

Recognizes human CXCR4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes: M. Loetscher, et al.; J. Biol. Chem. 269: 232 (1994). 2. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry: C.C. Bleul, et al.; Nature 382: 829 (1996)1. Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes: M. Loetscher, et al.; J. Biol. Chem. 269: 232 (1994). 2. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry: C.C. Bleul, et al.; Nature 382: 829 (1996)