Technical Data
C8376-25
CXCR7 (C-X-C Chemokine Receptor 7, Chemokine Orphan Receptor 1, RDC-1)
Description:
The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96% and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using C8376-26), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types. The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 25ug/ml. Use 10ul to label 1-2.5x10e5 cells in a total reaction volume 200ul.
Immunohistochemistry (Paraffin): 8-25ug/ml detected CXCR7 in paraffin sections of nude mice injected with human breast cancer cells.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse myeloma fused with B cells obtained from a mouse immunized with NS0 cells transfected with human CXCR7.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
TypeIsotypeCloneGrade
MabIgG2a7H134Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Human CXCR7 expressed in NSO cells (P25106).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes human CXCR7. Reacts specifically as detected by Flow Cytometry with five distinct human CXCR7 transfectants, but not their respective parental lines. Also reacts with monocytes expression of CXCR7. Does not react with MCF-7 cells by Flow Cytometry, which have been reported to have surface-expressing CXCR7 using C8376-26. Due to the conflicting reports published, use of monoclonal C8376-25 may result in an underestimation of CXCR7 expression on certain cell types.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. J. Biol. Chem. 2005, 280(42):35760. 2. J. Immunol. 2006, 176(4):2197. 3. J. Exp. Med. 2006, 203(9):2201