Technical Data
C8385-01
Cyan Fluorescent Protein (CFP)
Description:
The recombinant CFP (Cyan Fluorescent Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal CFP fluorescence. The protein is suitable as a control reagent for CFP expression studies or as a labeling reagent. Applications include: Use as standards for SDS-PAGE, Western blot analysis of CFP transfected cells, label other proteins, calibration of fluorometers and flow cytometers, fluorescence microscope, or microinjection of CFP into cells and tissues, etc.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5-4 ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
C8385-01A: Cyan Fluorescent Protein, Recombinant

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRabbit
Concentration:
~0.5mg/ml
Immunogen:
E. coli expressed Cyan Fluorescent Protein (CFP).
Purity:
Purified by affinitiy chromatography
Form
Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Specificity:
Recognizes Cyan Fluorescent Protein (CFP). Crossrectivity: Has a minimum (<5%) crossreactivity with Yellow fluorescent protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.