Technical Data
C8450-07D
cAMP (Cyclic Adenosine Monophosphate, Cyclic AMP, Adenosine 3'5'-cyclic Monophosphate) (HRP)
500ul
Biochemicals Storage: -20CShipping: Blue Ice
Cyclic Adenosine Monophosphate is a hydrophilic secondary messenger that is part of the cellular signal transduction pathway. It is produced by enzymatic methods on the molecule ATP by the enzyme adenylyl cyclase. It interacts with receptor proteins on the surface of a cell membrane following an intracellular signal produced by hormones or neurotransmitters. Cyclic AMP activates Protein Kinase A (PKA) catalytic ability. This activated PKA molecule transfers phosphate from ATP to different amino acid sequences. These phosphorylated amino acids can regulate calcium ion channels allowing calcium to move across the cellular membrane.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
ELISA (Competitive): 1:80
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Dilute as required and use the working strength conjugate immediately after dilution. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Concentration: ~5mg/ml
Form: Supplied as a liquid in PBS, pH 7.2. Labeled with HRP.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.