Technical Data
C8452-08
Cyclin A
Description:
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml detects p58 Cyclin A in HeLa nuclear extract. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with 2ug/ml C8452-08. Proteins were visualized using goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Immunoprecipitation: 1-5ug/ml. The precipitate may be used to perform a histone H1 kinase assay.

Immunocytochemistry: 10ug/ml

Optimal dilution to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Synthetic peptide (KLH-coupled) corresponding to aa 417-432 (C-KHGVSLLNPPETLNL) within the C-terminus of human p58 cyclin A
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O or 40-50% glycerol for long-term storage.
Specificity:
Recognizes human p58 cyclin A. Does not crossreact with cyclin B or cyclin D. Species Crossreactivity: Mouse, rat and sheep
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Wang, et. al., Nature 343: 555 (1990)
Hall, et al., J. Biol. Chem. 266: 17430 (1991)
Williams, et al., Oncogene 7: 423 (1992)
Hall, et al., J. Biol. Chem. 266: 17430-17440 (1991)
Williams, et al., Oncogene 7: 423-432 (1992)
Hall, et al., Oncogene 8: 1377-1384 (1993)
Carbonaro-Hall, et al., Oncogene 8: 1649-1659 (1993)