Technical Data
C8452-08K
Cyclin A, CT
Description:
Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's). They are expressed temporally to control transition at different specific phases of the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G-phase.

Applications:
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 5ug/ml for 2h, room temperature
Immunoprecipitation: 10ug/mg protein lysate
Immunohistochemistry: 1:50 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.

Positive Control:
Hela cells, tonsils

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to human cyclin A, at C-terminal.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Specificity:
Recognizes human cyclin A. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.