Technical Data
C8452-38
Cyclin A
Description:
In eukaroytic cells, the cell cycle is mediated by the sequential, activation and inactivation of complexes containing cyclin dependent kinases (CDKs) and cyclins. A number of CDKs have been identified and the activity of these various CDK family members is regulated by multiple mechanisms including binding to specific cyclin proteins, binding to specific small molecular weight inhibitory protein, and reversible phosphorylation. Binding of different cyclin protein is required to activate the catalytically inactive CDKs and determines the substrate specificity of the kinase. On the other hand, CDK inhibitors exert their inhibitory effect by blocking the catalytic activity of the cyclin-bound CDKs. Since the concentration of the CDKs are in excess and remain fairly constant throughout the cell cycle, their activation is determined by the availability of the different cyclin proteins and inhibitory factors.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 5-10ug/ml detects Cyclin A in bovine, human, monkey and rat
ELISA: 0.1-1ug/ml
Western Blot: 1-2ug/ml detects Cyclin A in A431 cell lysate, bovine, HeLa cell lysate, human, monkey and mouse. Very sensitive for active Cyclin A protein.
Immunoprecipitation: 2.5-5ug/ml detects Cyclin A in bovine, human, monkey and mouse
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k3G111Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceBovineMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant bovine Cyclin A protein
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Speci c for the 60kD cyclin A protein and can be utilized to detect human, monkey, mouse and bovine cyclin A. Crossreactivity with other cyclin family members has not been detected.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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