Technical Data
C8452-38
Cyclin A
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Technical Data |
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C8452-38 |
Cyclin A |
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Description: In eukaroytic cells, the cell cycle is mediated by the sequential, activation and inactivation of complexes containing cyclin dependent kinases (CDKs) and cyclins. A number of CDKs have been identified and the activity of these various CDK family members is regulated by multiple mechanisms including binding to specific cyclin proteins, binding to specific small molecular weight inhibitory protein, and reversible phosphorylation. Binding of different cyclin protein is required to activate the catalytically inactive CDKs and determines the substrate specificity of the kinase. On the other hand, CDK inhibitors exert their inhibitory effect by blocking the catalytic activity of the cyclin-bound CDKs. Since the concentration of the CDKs are in excess and remain fairly constant throughout the cell cycle, their activation is determined by the availability of the different cyclin proteins and inhibitory factors. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunofluorescence: 5-10ug/ml detects Cyclin A in bovine, human, monkey and rat ELISA: 0.1-1ug/ml Western Blot: 1-2ug/ml detects Cyclin A in A431 cell lysate, bovine, HeLa cell lysate, human, monkey and mouse. Very sensitive for active Cyclin A protein. Immunoprecipitation: 2.5-5ug/ml detects Cyclin A in bovine, human, monkey and mouse Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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